Title : Application of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters - Dos Santos_2020_Biotechnol.Appl.Biochem__ |
Author(s) : Dos Santos MMO , Gama RS , de Carvalho Tavares IM , Santos PH , Goncalves MS , de Carvalho MS , de Barros Vilas Boas EV , de Oliveira JR , Mendes AA , Franco M |
Ref : Biotechnol Appl Biochem , : , 2020 |
Abstract :
The present study aimed at preparing three biocatalysts via physical adsorption of lipases from Candida rugosa (CRL), Mucor javanicus (MJL), and Candida sp. (CALA) on a hydrophobic and mesoporous support (Diaion HP-20). These biocatalysts were later applied to the synthesis of aromatic esters of apple peel and citrus (hexyl butyrate), apple and rose (geranyl butyrate), and apricot and pineapple (propyl butyrate). Scanning electron microscopy (SEM) and gel electrophoresis confirmed a selective adsorption of lipases on Diaion, thus endorsing simultaneous immobilization and purification. Gibbs free energy (G) evinced the spontaneity of the process (-17.9 kJ/mol = G = -5.1 kJ/mol). Maximum immobilized protein concentration of 30 mg/g support by CRL. This biocatalyst was the most active in olive oil hydrolysis (hydrolytic activity of 126.0 +/- 2.0 U/g) and in the synthesis of aromatic esters. Maximum conversion yield of 89.1% was attained after 150 min for the synthesis of hexyl butyrate, followed by the synthesis of geranyl butyrate (87.3% after 240 min) and propyl butyrate (80.0% after 150 min). CRL immobilized on Diaion retained around 93% of its original activity after 6 consecutive cycles of 150 min for the synthesis of hexyl butyrate. This article is protected by copyright. All rights reserved. |
PubMedSearch : Dos Santos_2020_Biotechnol.Appl.Biochem__ |
PubMedID: 32438471 |
Dos Santos MMO, Gama RS, de Carvalho Tavares IM, Santos PH, Goncalves MS, de Carvalho MS, de Barros Vilas Boas EV, de Oliveira JR, Mendes AA, Franco M (2020)
Application of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters
Biotechnol Appl Biochem
:
Dos Santos MMO, Gama RS, de Carvalho Tavares IM, Santos PH, Goncalves MS, de Carvalho MS, de Barros Vilas Boas EV, de Oliveira JR, Mendes AA, Franco M (2020)
Biotechnol Appl Biochem
: