Dos Santos_2023_Food.Sci.Biotechnol_32_689

This study aims at the synthesis of hexyl butyrate by Candida rugosa lipase (CRL) immobilized on Diaion HP 20. The lipase load used was 28.7 +/- 2.1 mg/g (mg of lipase/g of support), whose hydrolytic activity was 132.0 +/- 2.5 U/g. To obtain the maximum production of hexyl butyrate, the Box-Behnken design statistical planning was used, having as independent variables; biocatalyst concentration, temperature and acid:alcohol molar ratio and ester conversion as a dependent variable at 60, 180 and 480 min. For 60 min, 90.8% conversion was obtained at 47.25 degC, 1:1.4 molar ratio and 17.65% of biocatalyst; 180 min, 94.5% conversion at 59.5 degC, 1:2 molar ratio and 15.8% biocatalyst; 480 min, 95.01% conversion at 47.0 degC, 1:2 molar ratio and 16.9% biocatalyst. CRL-Diaion HP 20 retained 60% of its initial activity after ten cycles of reactions showing potential for industrial use. The ester produced was identified by gas chromatography analyses. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10068-022-01200-1.