Droce_2016_Molecules_21_1710

Reference

Title : Functional Analysis of the Fusarielin Biosynthetic Gene Cluster - Droce_2016_Molecules_21_1710
Author(s) : Droce A , Saei W , Jorgensen SH , Wimmer R , Giese H , Wollenberg RD , Sondergaard TE , Sorensen JL
Ref : Molecules , 21 : , 2016
Abstract :

Fusarielins are polyketides with a decalin core produced by various species of Aspergillus and Fusarium. Although the responsible gene cluster has been identified, the biosynthetic pathway remains to be elucidated. In the present study, members of the gene cluster were deleted individually in a Fusarium graminearum strain overexpressing the local transcription factor. The results suggest that a trans-acting enoyl reductase (FSL5) assists the polyketide synthase FSL1 in biosynthesis of a polyketide product, which is released by hydrolysis by a trans-acting thioesterase (FSL2). Deletion of the epimerase (FSL3) resulted in accumulation of an unstable compound, which could be the released product. A novel compound, named prefusarielin, accumulated in the deletion mutant of the cytochrome P450 monooxygenase FSL4. Unlike the known fusarielins from Fusarium, this compound does not contain oxygenized decalin rings, suggesting that FSL4 is responsible for the oxygenation.

PubMedSearch : Droce_2016_Molecules_21_1710
PubMedID: 27983606
Gene_locus related to this paper: gibze-fsl2

Related information

Gene_locus gibze-fsl2

Citations formats

Droce A, Saei W, Jorgensen SH, Wimmer R, Giese H, Wollenberg RD, Sondergaard TE, Sorensen JL (2016)
Functional Analysis of the Fusarielin Biosynthetic Gene Cluster
Molecules 21 :

Droce A, Saei W, Jorgensen SH, Wimmer R, Giese H, Wollenberg RD, Sondergaard TE, Sorensen JL (2016)
Molecules 21 :