| Title : Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors - Dubovy_1999_Gen.Physiol.Biophys_18 Suppl 1_63 |
| Author(s) : Dubovy P , Svizenska I , Jancalek R , Klusakova I , Houstava L , Haninec P , Zitkova A |
| Ref : Gen Physiol Biophys , 18 Suppl 1 :63 , 1999 |
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Abstract :
The presence of laminin-1, collagen-IV, alpha6 and beta1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-1-, beta1-, and alpha6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells. |
| PubMedSearch : Dubovy_1999_Gen.Physiol.Biophys_18 Suppl 1_63 |
| PubMedID: 10707839 |
Dubovy P, Svizenska I, Jancalek R, Klusakova I, Houstava L, Haninec P, Zitkova A (1999)
Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
Gen Physiol Biophys
18 Suppl 1 :63
Dubovy P, Svizenska I, Jancalek R, Klusakova I, Houstava L, Haninec P, Zitkova A (1999)
Gen Physiol Biophys
18 Suppl 1 :63