| Title : Specific antibodies to bovine choline acetyltransferase raised in mice immunised with small amounts of partially purified enzyme - Eder-Colli_1982_Neurochem.Int_4_383 |
| Author(s) : Eder-Colli L , Powell JF , Cuello AC , Smith AD |
| Ref : Neurochem Int , 4 :383 , 1982 |
|
Abstract :
Choline acetyltransferase was purified approximately 18,000-fold from 300 g of bovine caudate nuclei to a specific activity of 21 ?mol min mg protein. The overall procedure used was: extraction of the enzyme by high salt concentration, chromatography on carboxy-methyl-Sephadex, precipitation by ammonium sulphate, affinity chromatography on Blue-Sepharose and, finally absorption on hydroxylapatite. When the enzyme absorbed on hydroxylapatite was injected into mice, it provoked reproducibly a transient production of 'inhibitory' antibodies, followed by higher antibody titres mainly of 'non-inhibitory' type. These responses were elicited by injecting less than a total of 20 ?g of immunogen. The highest antibody titre was obtained less than 2 months following the initial immunisation. Species cross reactivity was investigated. This procedure should prove to be of value in the production of monoclonal antibodies to choline acetyltransferase. |
| PubMedSearch : Eder-Colli_1982_Neurochem.Int_4_383 |
| PubMedID: 20487891 |
Eder-Colli L, Powell JF, Cuello AC, Smith AD (1982)
Specific antibodies to bovine choline acetyltransferase raised in mice immunised with small amounts of partially purified enzyme
Neurochem Int
4 :383
Eder-Colli L, Powell JF, Cuello AC, Smith AD (1982)
Neurochem Int
4 :383