Edin_2023_J.Biol.Chem__103049

Cytochromes P450 (CYP) metabolize arachidonic acid (AA) to epoxyeicosatrienoic acids (EETs) which have numerous effects. After cardiac ischemia, EET-induced coronary vasodilation increases delivery of oxygen/nutrients to the myocardium, and EET-induced signaling protects cardiomyocytes against post-ischemic mitochondrial damage. Soluble epoxide hydrolase 2 (EPHX2) diminishes the benefits of EETs through hydrolysis to less active dihydroxyeicosatrienoic acids (DHETs). EPHX2 inhibition or genetic disruption improves recovery of cardiac function after ischemia. Immunohistochemical staining revealed EPHX2 expression in cardiomyocytes and some endothelial cells, but little expression in cardiac smooth muscle cells or fibroblasts. To determine specific roles of EPHX2 in cardiac cell types, we generated mice with cell-specific disruption of Ephx2 in endothelial cells (Ephx2(fx/fx)/Tek-cre) or cardiomyocytes (Ephx2(fx/fx)/Myh6-cre) to compare to global Ephx2-deficient mice (global Ephx2(-/-)) and wild-type (Ephx2(fx/fx)) mice in expression, EET hydrolase activity, and heart function studies. Most cardiac EPHX2 expression and activity is in cardiomyocytes with substantially less in endothelial cells. Ephx2(fx/fx)/Tek-cre hearts have similar EPHX2 expression, hydrolase activity, and post-ischemic cardiac function as control Ephx2(fx/fx) hearts. However, Ephx2(fx/fx)/Myh6-cre hearts were similar to global Ephx2(-/-) hearts with significantly diminished EPHX2 expression, decreased hydrolase activity, and enhanced post-ischemic cardiac function compared to Ephx2(fx/fx) hearts. During reperfusion, Ephx2(fx/fx)/Myh6-cre hearts displayed increased ERK activation compared to Ephx2(fx/fx) hearts which could be reversed by EEZE treatment. EPHX2 did not regulate coronary vasodilation in this model. We conclude that EPHX2 is primarily expressed in cardiomyocytes where it regulates EET hydrolysis and post-ischemic cardiac function, whereas endothelial EPHX2 does not play a significant role in these processes.