El Sayed_2020_Biomed.Pharmacother_127_110143

As ongoing investigation of Huernia saudi-arabica D.V.Field (Asclepiadaceae), a new steroidal pregnane glycoside (Huernioside A) was isolated from dichloromethane fraction (DCM); it was identified as 3beta, 11, 14beta, 20(R)-tetrahydroxy-pregna-5,9(11)-diene-3-O-beta-D-thevetopyranosyl-(1-4)-beta-D- cymaropyranoside(HCP) through analysis of 1D, 2D NMR besides ESI-MS data. The alcoholic extract of the aerial part (ALE), DCM and HCP showed inhibitory potential against pancreatic lipase compared to orilstat. Among the tested samples, the ALE and HCP exhibited a promising pancreatic lipase inhibitory commotion through IC50 values of 0.61+/-0.15, 1.23+/-0.07mg/ml (equivalent to 88.8 muM), respectively. HCP was prevailed to have a mixed mode of inhibition as exposed by enzyme kinetic studies. Hydrophobic interactions were the major forces involved in ligand enzyme interactions. In contrast, moderate alpha-glucosidase inhibitory activities were evidenced for ALE and HCP (% inhibition: 24.8+/-1.8 and 26.6+/-2.5, respectively) compared to acarbose. This investigation is the first to report on the possible in vitro anti-obesity and anti-diabetic impact of H. saudi-arabica.