Elsemore_1994_J.Bacteriol_176_7659

Reference

Title : The pca-pob supraoperonic cluster of Acinetobacter calcoaceticus contains quiA, the structural gene for quinate-shikimate dehydrogenase - Elsemore_1994_J.Bacteriol_176_7659
Author(s) : Elsemore DA , Ornston LN
Ref : Journal of Bacteriology , 176 :7659 , 1994
Abstract :

An 18-kbp Acinetobacter calcoaceticus chromosomal segment contains the pcaIJFBDKCHG operon, which is required for catabolism of protocatechuate, and pobSRA, genes associated with conversion of p-hydroxybenzoate to protocatechuate. The genetic function of the 6.5 kbp of DNA between pcaG and pobS was unknown. Deletions in this DNA were designed by removal of fragments between restriction sites, and the deletion mutations were introduced into A. calcoaceticus by natural transformation. The mutations prevented growth with either quinate or shikimate, growth substrates that depend upon qui gene function for their catabolism to protocatechuate. The location of quiA, a gene encoding quinate-shikimate dehydrogenase, was indicated by its expression in one of the deletion mutants, and the position of the gene was confirmed by determination of its 2,427-bp nucleotide sequence. The deduced amino acid sequence of QuiA confirmed that it is a member of a family of membrane-associated, pyrrolo-quinoline quinone-dependent dehydrogenases, as had been suggested by earlier biochemical investigations. Catabolism of quinate and skikimate is initiated by NAD(+)-dependent dehydrogenases in other microorganisms, so it is evident that different gene pools were called upon to provide the ancestral enzyme for this metabolic step.

PubMedSearch : Elsemore_1994_J.Bacteriol_176_7659
PubMedID: 8002591
Gene_locus related to this paper: aciad-q8rlz8 , acica-elh1

Related information

Gene_locus aciad-q8rlz8    acica-elh1

Citations formats

Elsemore DA, Ornston LN (1994)
The pca-pob supraoperonic cluster of Acinetobacter calcoaceticus contains quiA, the structural gene for quinate-shikimate dehydrogenase
Journal of Bacteriology 176 :7659

Elsemore DA, Ornston LN (1994)
Journal of Bacteriology 176 :7659