| Title : New efficient recombinant expression system to engineer Candida antarctica lipase B - Emond_2010_Appl.Environ.Microbiol_76_2684 |
| Author(s) : Emond S , Montanier C , Nicaud JM , Marty A , Monsan P , Andre I , Remaud-Simeon M |
| Ref : Applied Environmental Microbiology , 76 :2684 , 2010 |
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Abstract :
Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme. |
| PubMedSearch : Emond_2010_Appl.Environ.Microbiol_76_2684 |
| PubMedID: 20173074 |
Emond S, Montanier C, Nicaud JM, Marty A, Monsan P, Andre I, Remaud-Simeon M (2010)
New efficient recombinant expression system to engineer Candida antarctica lipase B
Applied Environmental Microbiology
76 :2684
Emond S, Montanier C, Nicaud JM, Marty A, Monsan P, Andre I, Remaud-Simeon M (2010)
Applied Environmental Microbiology
76 :2684