Title : A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay - Fabritz_2012_AMB.Express_2_51 |
Author(s) : Fabritz S , Maass F , Avrutina O , Heiseler T , Steinmann B , Kolmar H |
Ref : AMB Express , 2 :51 , 2012 |
Abstract :
ABSTRACT A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described It is based on a multistage enzyme reaction which allows for the analysis of alkyl halides in buffered systems Irreversible hydrolytic dehalogenation catalyzed by haloalkane dehalogenase DhaA from Rhodococcus erythropolis transfers an alkyl halide into a corresponding alcohol that is further oxidized by alcohol oxidase AOX from Pichia pastoris yielding a respective aldehyde and hydrogen peroxide easily detectable via the horseradish peroxidase catalyzed oxidation of chromogenic molecules Due to its high sensitivity 0.025 mM 0.43 ppm for 1,3-dibromopropane low expenditure and the ability of handling a large number of samples in parallel this method is an attractive alternative to existing procedures for the monitoring of both haloalkanes and dehalogenases. |
PubMedSearch : Fabritz_2012_AMB.Express_2_51 |
PubMedID: 23006907 |
Substrate | 1,3-dibromopropane |
Fabritz S, Maass F, Avrutina O, Heiseler T, Steinmann B, Kolmar H (2012)
A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay
AMB Express
2 :51
Fabritz S, Maass F, Avrutina O, Heiseler T, Steinmann B, Kolmar H (2012)
AMB Express
2 :51