| Title : Acetylcholinesterase inhibition, antioxidant activity and toxicity of Peumus boldus water extracts on HeLa and Caco-2 cell lines - Fale_2012_Food.Chem.Toxicol_50_2656 |
| Author(s) : Fale PL , Amaral F , Amorim Madeira PJ , Sousa Silva M , Florencio MH , Frazao FN , Serralheiro ML |
| Ref : Food & Chemical Toxicology , 50 :2656 , 2012 |
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Abstract :
This work aimed to study the inhibition on acetylcholinesterase activity (AChE), the antioxidant activity and the toxicity towards Caco-2 and HeLa cells of aqueous extracts of Peumus Boldus. An IC(50) value of 0.93 mg/mL, for AChE inhibition, and EC(50) of 18.7 mug/mL, for the antioxidant activity, was determined. This activity can be attributed to glycosylated flavonoid derivatives detected, which were the main compounds, although boldine and other aporphine derivatives were also present. No changes in the chemical composition or the biochemical activities were found after gastrointestinal digestion. Toxicity of P. boldus decoction gave an IC(50) value 0.66 mg/mL for HeLa cells, which caused significant changes in the cell proteome profile. |
| PubMedSearch : Fale_2012_Food.Chem.Toxicol_50_2656 |
| PubMedID: 22617353 |
Fale PL, Amaral F, Amorim Madeira PJ, Sousa Silva M, Florencio MH, Frazao FN, Serralheiro ML (2012)
Acetylcholinesterase inhibition, antioxidant activity and toxicity of Peumus boldus water extracts on HeLa and Caco-2 cell lines
Food & Chemical Toxicology
50 :2656
Fale PL, Amaral F, Amorim Madeira PJ, Sousa Silva M, Florencio MH, Frazao FN, Serralheiro ML (2012)
Food & Chemical Toxicology
50 :2656