| Title : Quantal acetylcholine release induced by mediatophore transfection - Falk-Vairant_1996_Proc.Natl.Acad.Sci.U.S.A_93_5203 |
| Author(s) : Falk-Vairant J , Correges P , Eder-Colli L , Salem N , Roulet E , Bloc A , Meunier F , Lesbats B , Loctin F , Synguelakis M , Israel M , Dunant Y |
| Ref : Proc Natl Acad Sci U S A , 93 :5203 , 1996 |
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Abstract :
Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed in situ in cholinergic synapses from which mediatophore was purified. |
| PubMedSearch : Falk-Vairant_1996_Proc.Natl.Acad.Sci.U.S.A_93_5203 |
| PubMedID: 8643553 |
Falk-Vairant J, Correges P, Eder-Colli L, Salem N, Roulet E, Bloc A, Meunier F, Lesbats B, Loctin F, Synguelakis M, Israel M, Dunant Y (1996)
Quantal acetylcholine release induced by mediatophore transfection
Proc Natl Acad Sci U S A
93 :5203
Falk-Vairant J, Correges P, Eder-Colli L, Salem N, Roulet E, Bloc A, Meunier F, Lesbats B, Loctin F, Synguelakis M, Israel M, Dunant Y (1996)
Proc Natl Acad Sci U S A
93 :5203