| Title : Aii810, a Novel Cold-Adapted N-Acylhomoserine Lactonase Discovered in a Metagenome, Can Strongly Attenuate Pseudomonas aeruginosa Virulence Factors and Biofilm Formation - Fan_2017_Front.Microbiol_8_1950 |
| Author(s) : Fan X , Liang M , Wang L , Chen R , Li H , Liu X |
| Ref : Front Microbiol , 8 :1950 , 2017 |
| Abstract : The pathogen Pseudomonas aeruginosa uses quorum sensing (QS) to control virulence and biofilm formation. Enzymatic disruption of quorum sensing is a promising anti-infection therapeutic strategy that does not rely on antibiotics. Here, a novel gene (aii810) encoding an N-acylhomoserine lactonase was isolated from the Mao-tofu metagenome for the first time. Aii810 encoded a protein of 269 amino acids and was expressed in Escherichia coli BL21 (DE3) in soluble form. It showed the highest activity at 20 degrees C, and it maintained 76.5% of activity at 0 degrees C and more than 50% activity at 0-40 degrees C. The optimal pH was 8.0. It was stable in both neutral and slightly alkaline conditions and at temperatures below 40 degrees C. The enzyme hydrolyzed several rho-nitrophenyl esters, but its best substrate was rho-nitrophenyl acetate. Its kcat and Km values were 347.7 S(-1) and 205.1 muM, respectively. It efficiently degraded N-butyryl-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone, exceeding hydrolysis rates of 72.3 and 100%, respectively. Moreover, Aii810 strongly attenuated P. aeruginosa virulence and biofilm formation. This enzyme with high anti-QS activity was the most cold-adapted N-acylhomoserine lactonase reported, which makes it an attractive enzyme for use as a therapeutic agent against P. aeruginosa infection. |
| ESTHER : Fan_2017_Front.Microbiol_8_1950 |
| PubMedSearch : Fan_2017_Front.Microbiol_8_1950 |
| PubMedID: 29067011 |
| Gene_locus related to this paper: 9bact-Aii810 |
| Gene_locus related to this paper: 9bact-Aii810 |
Fan X, Liang M, Wang L, Chen R, Li H, Liu X (2017)
Aii810, a Novel Cold-Adapted N-Acylhomoserine Lactonase Discovered in a Metagenome, Can Strongly Attenuate Pseudomonas aeruginosa Virulence Factors and Biofilm Formation
Front Microbiol
8 :1950
Fan X, Liang M, Wang L, Chen R, Li H, Liu X (2017)
Front Microbiol
8 :1950