Fan_2023_Molecules_28_6738

Reference

Title : A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism - Fan_2023_Molecules_28_6738
Author(s) : Fan S , Guo J , Han S , Du H , Wang Z , Fu Y , Han H , Hou X , Wang W
Ref : Molecules , 28 :6738 , 2023
Abstract :

Phthalic acid esters (PAEs), which are widespread environmental contaminants, can be efficiently biodegraded, mediated by enzymes such as hydrolases. Despite great advances in the characterization of PAE hydrolases, which are the most important enzymes in the process of PAE degradation, their molecular catalytic mechanism has rarely been systematically investigated. Acinetobacter sp. LUNF3, which was isolated from contaminated soil in this study, demonstrated excellent PAE degradation at 30 degreesC and pH 5.0-11.0. After sequencing and annotating the complete genome, the gene dphAN1, encoding a novel putative PAE hydrolase, was identified with the conserved motifs catalytic triad (Ser(201)-Asp(295)-His(325)) and oxyanion hole (H(127)GGG(130)). DphAN1 can hydrolyze DEP (diethyl phthalate), DBP (dibutyl phthalate) and BBP (benzyl butyl phthalate). The high activity of DphAN1 was observed under a wide range of temperature (10-40 degreesC) and pH (6.0-9.0). Moreover, the metal ions (Fe(2+), Mn(2+), Cr(2+) and Fe(3+)) and surfactant TritonX-100 significantly activated DphAN1, indicating a high adaptability and tolerance of DphAN1 to these chemicals. Molecular docking revealed the catalytic triad, oxyanion hole and other residues involved in binding DBP. The mutation of these residues reduced the activity of DphAN1, confirming their interaction with DBP. These results shed light on the catalytic mechanism of DphAN1 and may contribute to protein structural modification to improve catalytic efficiency in environment remediation.

PubMedSearch : Fan_2023_Molecules_28_6738
PubMedID: 37764514
Gene_locus related to this paper: 9gamm-a0a7h9sp49

Related information

Gene_locus 9gamm-a0a7h9sp49

Citations formats

Fan S, Guo J, Han S, Du H, Wang Z, Fu Y, Han H, Hou X, Wang W (2023)
A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism
Molecules 28 :6738

Fan S, Guo J, Han S, Du H, Wang Z, Fu Y, Han H, Hou X, Wang W (2023)
Molecules 28 :6738