| Title : Characterization of fluoroalcohols-induced intermediates of Mucor miehei lipase at low pH - Fatima_2008_Protein.Pept.Lett_15_346 |
| Author(s) : Fatima S , Mishra A , Sen P , Khan RH |
| Ref : Protein Pept Lett , 15 :346 , 2008 |
|
Abstract :
We have previously characterized an acid-unfolded (U(A)) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with beta-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. alpha-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP. |
| PubMedSearch : Fatima_2008_Protein.Pept.Lett_15_346 |
| PubMedID: 18473945 |
Fatima S, Mishra A, Sen P, Khan RH (2008)
Characterization of fluoroalcohols-induced intermediates of Mucor miehei lipase at low pH
Protein Pept Lett
15 :346
Fatima S, Mishra A, Sen P, Khan RH (2008)
Protein Pept Lett
15 :346