Fedon_1993_DNA.Seq_3_347

Reference

Title : cDNA sequence, gene structure, and cholinesterase-like domains of an esterase from Caenorhabditis elegans mapped to chromosome V - Fedon_1993_DNA.Seq_3_347
Author(s) : Fedon Y , Cousin X , Toutant JP , Thierry-Mieg D , Arpagaus M
Ref : DNA Sequence , 3 :347 , 1993
Abstract :

The structure of an esterase gene from Caenorhabditis elegans has been determined by comparison of the sequences in genomic and cDNA clones. The gene was mapped close to the center of chromosome V (1.7 centimorgans to the left of dpy-11) and is therefore distinct from the gut esterase gene ges-1. It possessed 7 short introns. The 5' splice site of intron 3 presented the sequence GC instead of the usual GT that was found in the other six introns. The cDNA was trans-spliced with the short leader SL1. The open reading frame indicated that a protein of 557 aminoacids was encoded. The deduced aminoacid sequence did not present a signal peptide at the N-terminal but a potential N-myristoylation site (GXXXS) provided that the initiator methionine was removed. This protein should therefore remain intracellular. Comparison of this C. elegans sequence to other protein sequences in databases, as well as the analysis of the secondary structure in the protein showed that it belongs to the subgroup of esterases in the alpha/beta hydrolase fold family.

PubMedSearch : Fedon_1993_DNA.Seq_3_347
PubMedID: 8219278
Gene_locus related to this paper: caeel-ester

Related information

Gene_locus caeel-ester
Family caeel-ester    Carb_B_Nematoda

Citations formats

Fedon Y, Cousin X, Toutant JP, Thierry-Mieg D, Arpagaus M (1993)
cDNA sequence, gene structure, and cholinesterase-like domains of an esterase from Caenorhabditis elegans mapped to chromosome V
DNA Sequence 3 :347

Fedon Y, Cousin X, Toutant JP, Thierry-Mieg D, Arpagaus M (1993)
DNA Sequence 3 :347