Title : Activity and stability of lipase from Candida Antarctica after treatment in pressurized fluids - Feiten_2023_Biotechnol.Lett__ |
Author(s) : Feiten MC , Morigi I , Di Luccio M , Oliveira JV |
Ref : Biotechnol Lett , : , 2023 |
Abstract :
Lipase B from Candida antarctica (CalB) is one of the biocatalysts most used in organic synthesis due to its ability to act in several medium, wide substrate specificity and enantioselectivity, tolerance to non-aqueous environment, and resistance to thermal deactivation. Thus, the objective of this work was to treat CalB in supercritical carbon dioxide (SC-CO(2)) and liquefied petroleum gas (LPG), and measure its activity before and after high-pressure treatment. Residual specific hydrolytic activities of 132% and 142% were observed when CalB was exposed to SC-CO(2) at 35s degC, 75sbar and 1sh and to LPG at 65s degC, 30sbar and 1sh, respectively. Residual activity of the enzyme treated at high pressure was still above 100% until the 20th day of storage at low temperatures. There was no difference on the residual activity loss of CalB treated with LPG and stored at different temperatures over time. Greater difference was observed between CalB treated with CO(2) and flash-frozen in liquid nitrogen (-s196s degC) followed by storage in freezer (-s10s degC) and CalB stored in freezer at -s10s degC. Such findings encourage deeper studies on CalB as well as other enzymes behavior under different types of pressurized fluids aiming at industrial application. |
PubMedSearch : Feiten_2023_Biotechnol.Lett__ |
PubMedID: 36592260 |
Feiten MC, Morigi I, Di Luccio M, Oliveira JV (2023)
Activity and stability of lipase from Candida Antarctica after treatment in pressurized fluids
Biotechnol Lett
:
Feiten MC, Morigi I, Di Luccio M, Oliveira JV (2023)
Biotechnol Lett
: