Felix_2019_Sci.Rep_9_13144

Reference

Title : A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes - Felix_2019_Sci.Rep_9_13144
Author(s) : Felix C , Meneses R , Goncalves MFM , Tilleman L , Duarte AS , Jorrin-Novo JV , Van de Peer Y , Deforce D , Van Nieuwerburgh F , Esteves AC , Alves A
Ref : Sci Rep , 9 :13144 , 2019
Abstract :

Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 degreesC and 37 degreesC were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 degreesC. At 37 degreesC, proteins related to pathogenicity were less abundant than at 25 degreesC, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 degreesC. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals.

PubMedSearch : Felix_2019_Sci.Rep_9_13144
PubMedID: 31511626
Gene_locus related to this paper: 9pezi-a0a5n5dna6 , 9pezi-a0a5n5di12 , 9pezi-a0a5n5d4q0

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Citations formats

Felix C, Meneses R, Goncalves MFM, Tilleman L, Duarte AS, Jorrin-Novo JV, Van de Peer Y, Deforce D, Van Nieuwerburgh F, Esteves AC, Alves A (2019)
A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes
Sci Rep 9 :13144

Felix C, Meneses R, Goncalves MFM, Tilleman L, Duarte AS, Jorrin-Novo JV, Van de Peer Y, Deforce D, Van Nieuwerburgh F, Esteves AC, Alves A (2019)
Sci Rep 9 :13144