Fendri_2014_J.Mol.Graph.Model_56C_1

Reference

Title : Cloning and molecular modeling of a thermostable carboxylesterase from the chicken uropygial glands - Fendri_2014_J.Mol.Graph.Model_56C_1
Author(s) : Fendri A , Frikha F , Louati H , Ali MB , Gargouri H , Gargouri Y , Miled N
Ref : J Mol Graph Model , 56C :1 , 2014
Abstract :

Starting from total uropygial glands mRNAs, chicken uropygial carboxylesterase (cuCES) cDNA was synthesized by RT-PCR and cloned into the PGEM-T vector. Amino acid sequence of the cuCES is compared to that of human liver carboxylesterase 1 (hCES1). Given the high amino acid sequence homology between the two enzymes, a 3-D structure model of the chicken carboxylesterase was built using the structure of hCES1 as template. By following this model and utilizing molecular dynamics (MD) simulations, the resistance of the chicken carboxylesterase at high temperatures could be explained. The docking of substrate analogs into the cuCES active site was used to explain the fact that the chicken carboxylesterase cannot hydrolyze efficiently large substrate molecules.

PubMedSearch : Fendri_2014_J.Mol.Graph.Model_56C_1
PubMedID: 25541525

Related information

Citations formats

Fendri A, Frikha F, Louati H, Ali MB, Gargouri H, Gargouri Y, Miled N (2014)
Cloning and molecular modeling of a thermostable carboxylesterase from the chicken uropygial glands
J Mol Graph Model 56C :1

Fendri A, Frikha F, Louati H, Ali MB, Gargouri H, Gargouri Y, Miled N (2014)
J Mol Graph Model 56C :1