Feng_1997_Appl.Environ.Microbiol_63_1332

Reference

Title : Plasmid-mediated mineralization of carbofuran by Sphingomonas sp. strain CF06 - Feng_1997_Appl.Environ.Microbiol_63_1332
Author(s) : Feng X , Ou LT , Ogram A
Ref : Applied Environmental Microbiology , 63 :1332 , 1997
Abstract :

A bacterial strain (CF06) that mineralized both the carbonyl group and the aromatic ring of the insecticide carbofuran and that is capable of using carbofuran as a sole source of carbon and nitrogen was isolated from a soil in Washington state. Phospholipid fatty acid and 16S rRNA sequencing analysis indicate that CF06 is a Sphingomonas sp. CF06 contains five plasmids, at least some of which are required for metabolism of carbofuran. Loss of the plasmids induced by growth at 42 degrees C resulted in the inability of the cured strain to grow on carbofuran as a sole source of carbon. Introduction of the plasmids confers on Pseudomonas fluorescens M480R the ability to use carbofuran as a sole source of carbon for growth and energy. Of the five plasmids, four are rich in insertion sequence elements and contain large regions of overlap. Rearrangements, deletions, and loss of individual plasmids that resulted in the loss of the carbofuran-degrading phenotype were observed following introduction of Tn5.

PubMedSearch : Feng_1997_Appl.Environ.Microbiol_63_1332
PubMedID: 9097429

Related information

Citations formats

Feng X, Ou LT, Ogram A (1997)
Plasmid-mediated mineralization of carbofuran by Sphingomonas sp. strain CF06
Applied Environmental Microbiology 63 :1332

Feng X, Ou LT, Ogram A (1997)
Applied Environmental Microbiology 63 :1332