| Title : Regions of beta 4.beta 2 subunit chimeras that contribute to the agonist selectivity of neuronal nicotinic receptors - Figl_1992_FEBS.Lett_308_245 |
| Author(s) : Figl A , Cohen BN , Quick MW , Davidson N , Lester HA |
| Ref : FEBS Letters , 308 :245 , 1992 |
|
Abstract :
Fifteen chimeric nicotinic receptor beta subunits were constructed consisting of N-terminal neuronal beta 4 sequences and C-terminal beta 2 sequences. Responses to cytisine, nicotine, or tetramethylammonium were compared to acetylcholine responses for these subunits expressed in Xenopus oocytes with alpha 3 subunits. The results show that (i) two residues in the extracellular domain of chimeric beta 4.beta 2 subunits (108 beta 2F/beta 4V, 110 beta 2S/beta 4T) account for much of the relative cytisine sensitivity; and (ii) four extracellular residues of chimeric beta 4.beta 2 subunits (112 beta 2A/beta 4V, 113 beta 2V/beta 4I and 115 beta 2S/beta 4R, 116 beta 2Y/beta 4S) account for most of the relative tetramethylammonium sensitivity. The data did not permit localization of nicotine sensitivity to any particular region. |
| PubMedSearch : Figl_1992_FEBS.Lett_308_245 |
| PubMedID: 1505662 |
Figl A, Cohen BN, Quick MW, Davidson N, Lester HA (1992)
Regions of beta 4.beta 2 subunit chimeras that contribute to the agonist selectivity of neuronal nicotinic receptors
FEBS Letters
308 :245
Figl A, Cohen BN, Quick MW, Davidson N, Lester HA (1992)
FEBS Letters
308 :245