| Title : N- and P-type Ca2+ channels are involved in acetylcholine release at a neuroneuronal synapse: only the N-type channel is the target of neuromodulators - Fossier_1994_Proc.Natl.Acad.Sci.U.S.A_91_4771 |
| Author(s) : Fossier P , Baux G , Tauc L |
| Ref : Proc Natl Acad Sci U S A , 91 :4771 , 1994 |
|
Abstract :
Cholinergic transmission in an identified neuro-neuronal synapse of the Aplysia buccal ganglion was depressed by application of a partially purified extract of the funnel-web-spider venom (FTx) or of its synthetic analog (sFTx). This specific blocker of voltage-dependent P-type Ca2+ channels did not interfere with the effect of the N-type Ca2+ channel blocker omega-conotoxin, which could further decrease synaptic transmission after a previous application of FTx. Similar results were obtained when the reversal order of application of these two Ca2+ channel blockers was used. Both P- and N-type Ca2+ currents trigger acetylcholine release in the presynaptic neuron. The neuromodulatory effects of FMRF-amide, histamine, and buccalin on transmitter release disappeared after the blockade of the N-type Ca2+ channels but remained still effective in the presence of FTx. These results indicate that only N-type Ca2+ channels appear to be sensitive to the neuromodulators we have identified. |
| PubMedSearch : Fossier_1994_Proc.Natl.Acad.Sci.U.S.A_91_4771 |
| PubMedID: 7910963 |
Fossier P, Baux G, Tauc L (1994)
N- and P-type Ca2+ channels are involved in acetylcholine release at a neuroneuronal synapse: only the N-type channel is the target of neuromodulators
Proc Natl Acad Sci U S A
91 :4771
Fossier P, Baux G, Tauc L (1994)
Proc Natl Acad Sci U S A
91 :4771