| Title : Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader - Galgani_1992_Ecotoxicol.Environ.Saf_23_237 |
| Author(s) : Galgani F , Cadiou Y , Gilbert F |
| Ref : Ecotoxicology & Environmental Safety , 23 :237 , 1992 |
|
Abstract :
A system is described for determination of LC50 or IC50 by an iterative process based on data obtained from a plate reader using a marine unicellular alga as a target species. The esterase activity of Tetraselmis suesica on fluorescein diacetate as a substrate was measured using a fluorescence titerplate. Simultaneous analysis of results was performed using an iterative process adopting the sigmoid function Y = y/1 (dose of toxicant/IC50)slope for dose-response relationships. IC50 (+/- SEM) was estimated (P less than 0.05). An application with phosalone as a toxicant is presented. |
| PubMedSearch : Galgani_1992_Ecotoxicol.Environ.Saf_23_237 |
| PubMedID: 1374329 |
Galgani F, Cadiou Y, Gilbert F (1992)
Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader
Ecotoxicology & Environmental Safety
23 :237
Galgani F, Cadiou Y, Gilbert F (1992)
Ecotoxicology & Environmental Safety
23 :237