| Title : Identification of an alkaline lipase capable of better enrichment of EPA than DHA due to fatty acids selectivity and regioselectivity - Gao_2020_Food.Chem_330_127225 |
| Author(s) : Gao K , Chu W , Sun J , Mao X |
| Ref : Food Chem , 330 :127225 , 2020 |
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Abstract :
The whole genome of Streptomyces violascens (=ATCC 27968) was sequenced and the cloning and expression of OUC-Lipase 6 were conducted in Bacillus subtilis WB800. The recombinant enzyme belongs to the lipolytic enzymes family V. OUC-Lipase 6 showed optimal activity at 30 degrees C and pH 9.0, and retained 90.2% of its activity in an alkaline buffer (pH 8.0, 30 degrees C and 96 h). OUC-Lipase 6 showed good stability under medium temperature conditions (residual activity of 68.8%, pH 8.0, 45 degrees C and 96 h). OUC-Lipase 6 could selectively hydrolyze fatty acids on the glyceride backbone, thus improving the contents of DHA and EPA in codfish oil. OUC-Lipase 6 also showed regioselectivity, resulting in a better enrichment efficiency for EPA than DHA. After hydrolyzing for 36 h via OUC-Lipase 6, the contents of EPA and DHA were improved to 3.24-fold and 1.98-fold, respectively. |
| PubMedSearch : Gao_2020_Food.Chem_330_127225 |
| PubMedID: 32569931 |
| Gene_locus related to this paper: 9actn-OUCLip6 |
| Gene_locus | 9actn-OUCLip6 |
Gao K, Chu W, Sun J, Mao X (2020)
Identification of an alkaline lipase capable of better enrichment of EPA than DHA due to fatty acids selectivity and regioselectivity
Food Chem
330 :127225
Gao K, Chu W, Sun J, Mao X (2020)
Food Chem
330 :127225