| Title : Detection of anti-Yta antibodies using a sensitive and specific enzyme-linked immunosorbent assay - Geen_1999_Br.J.Biomed.Sci_56_128 |
| Author(s) : Geen J , Hullin DA , Hogg SI |
| Ref : Br J Biomed Sci , 56 :128 , 1999 |
| Abstract : A specific, sensitive and semi-quantitative enzyme-linked immunosorbent assay (ELISA) is described to detect anti-Yta antibodies in human serum. Recombinant acetylcholinesterase (AChE E.C.3.1.1.7) was employed as the coating antigen in the microtitre plate and horseradish peroxidase (HRP)-conjugated specific antibody (IgG) was used as the secondary antibody. The method developed showed excellent sensitivity, detecting a titre > 1 in 600,000 (3.5 ng/mL mouse IgG protein) for mouse monoclonal (mMAb) anti-AChE antibody. No cross-reaction was seen with other common blood group antibodies, confirming the specificity of the method. The recombinant antigen's AChE phenotype was confirmed as Yta, as no reaction was detected with anti-Ytb-positive sera. The ELISA method correlated closely with the established serological grading system used routinely in blood transfusion laboratories. |
| ESTHER : Geen_1999_Br.J.Biomed.Sci_56_128 |
| PubMedSearch : Geen_1999_Br.J.Biomed.Sci_56_128 |
| PubMedID: 10695054 |
Geen J, Hullin DA, Hogg SI (1999)
Detection of anti-Yta antibodies using a sensitive and specific enzyme-linked immunosorbent assay
Br J Biomed Sci
56 :128
Geen J, Hullin DA, Hogg SI (1999)
Br J Biomed Sci
56 :128