| Title : Phylogenetic analysis of different isolates of Sulfobacillus spp. isolated from uranium-rich environments and recovery of genes using integron-specific primers - Ghauri_2003_Extremophiles_7_341 |
| Author(s) : Ghauri MA , Khalid AM , Grant S , Heaphy S , Grant WD |
| Ref : Extremophiles , 7 :341 , 2003 |
|
Abstract :
The isolation and phylogenetic characterization of acidophilic moderate thermophilic bacteria from different locations of uranium mines and a uranium processing mill in Pakistan is reported. The dominant culturable bacteria found were related to Sulfobacillus thermosulfidooxidans in all the samples analyzed. Different strains displayed different levels of identity (95-97%) to 16S rDNA of known strains of this species, indicating group heterogeneity. Genomic DNA from five isolates was subjected to amplification using integron-specific primers HS286 and HS287. Recovery of different integron-linked genes from one of the isolates indicated the usefulness of this approach for gene mining in place of traditional gene recovery methodologies. |
| PubMedSearch : Ghauri_2003_Extremophiles_7_341 |
| PubMedID: 12955603 |
Ghauri MA, Khalid AM, Grant S, Heaphy S, Grant WD (2003)
Phylogenetic analysis of different isolates of Sulfobacillus spp. isolated from uranium-rich environments and recovery of genes using integron-specific primers
Extremophiles
7 :341
Ghauri MA, Khalid AM, Grant S, Heaphy S, Grant WD (2003)
Extremophiles
7 :341