Title : Binding preferences for GPIHBP1, a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells - Gin_2011_Arterioscler.Thromb.Vasc.Biol_31_176 |
Author(s) : Gin P , Beigneux AP , Voss C , Davies BS , Beckstead JA , Ryan RO , Bensadoun A , Fong LG , Young SG |
Ref : Arterioscler Thromb Vasc Biol , 31 :176 , 2011 |
Abstract :
OBJECTIVE: To define the ability of GPIHBP1 to bind other lipase family members and other apolipoproteins (apos) and lipoproteins. METHODS AND RESULTS: GPIHBP1, a GPI-anchored lymphocyte antigen (Ly)6 protein of capillary endothelial cells, binds lipoprotein lipase (LPL) avidly, but its ability to bind related lipase family members has never been evaluated. As judged by cell-based and cell-free binding assays, LPL binds to GPIHBP1, but other members of the lipase family do not. We also examined the binding of apoAV-phospholipid disks to GPIHBP1. ApoAV binds avidly to GPIHBP1-transfected cells; this binding requires GPIHBP1's amino-terminal acidic domain and is independent of its cysteine-rich Ly6 domain (the latter domain is essential for LPL binding). GPIHBP1-transfected cells did not bind high-density lipoprotein. Chylomicrons bind avidly to GPIHBP1-transfected Chinese hamster ovary cells, but this binding is dependent on GPIHBP1's ability to bind LPL within the cell culture medium. CONCLUSIONS: GPIHBP1 binds LPL but does not bind other lipase family members. GPIHBP1 binds apoAV but does not bind apoAI or high-density lipoprotein. The ability of GPIHBP1-transfected Chinese hamster ovary cells to bind chylomicrons is mediated by LPL; chylomicron binding does not occur unless GPIHBP1 first captures LPL from the cell culture medium. |
PubMedSearch : Gin_2011_Arterioscler.Thromb.Vasc.Biol_31_176 |
PubMedID: 20966398 |
Gin P, Beigneux AP, Voss C, Davies BS, Beckstead JA, Ryan RO, Bensadoun A, Fong LG, Young SG (2011)
Binding preferences for GPIHBP1, a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells
Arterioscler Thromb Vasc Biol
31 :176
Gin P, Beigneux AP, Voss C, Davies BS, Beckstead JA, Ryan RO, Bensadoun A, Fong LG, Young SG (2011)
Arterioscler Thromb Vasc Biol
31 :176