Giompres_1984_Biochim.Biophys.Acta_770_166

In this study we demonstrate differences in the osmotic fragility of two metabolically and physically heterogeneous synaptic vesicle populations from stimulated electromotor nerve terminals. When synaptic vesicles isolated on sucrose density gradients are submitted to solutions of decreasing osmolarity 50% of VP2-type vesicles lysed at (mean + S.E. (number of experiments] 332 +/- 14 (4) mosM and 50% of VP1-type vesicles lysed at 573 +/- 8 (3) mosM. These results indicate that recycling vesicles are more resistant to hypo-osmotic lysis and they are consistent with our earlier conclusion that changes in water content on recycling are secondary to changes in the content of the osmotically active small-molecular-mass constituents acetylcholine and ATP.