| Title : Stimulation of a neutral cholesteryl ester hydrolase by cAMP system in P388D1 macrophages - Goldberg_1990_Biochim.Biophys.Acta_1042_132 |
| Author(s) : Goldberg DI , Khoo JC |
| Ref : Biochimica & Biophysica Acta , 1042 :132 , 1990 |
| Abstract : Cholesteryl ester laden foam cells in atherosclerotic lesions derive, in part, from macrophages. Mobilization of stored cholesteryl esters involves hydrolysis by a neutral cholesteryl ester hydrolase. Incubation of intact P388D1 macrophages with dibutyryl cAMP in the presence of 1-methyl-3-isobutylxanthine resulted in a dose-dependent increase in neutral cholesteryl ester hydrolase activity of up to 50% (ED50 = 0.1 mM). Incubation with prostaglandin E1 in the presence of 1-methyl-3-isobutylxanthine also increased neutral cholesterol ester hydrolase activity by about 50%. In cell-free preparation, cAMP-dependent protein kinase caused about a 2-fold activation of the neutral cholesteryl ester hydrolase. Activation was blocked by protein kinase inhibitor. These data suggest that the P388D1 macrophage may be a useful model for studying the hormonal regulation of cholesteryl ester mobilization in macrophage-derived foam cells. |
| ESTHER : Goldberg_1990_Biochim.Biophys.Acta_1042_132 |
| PubMedSearch : Goldberg_1990_Biochim.Biophys.Acta_1042_132 |
| PubMedID: 2153410 |
Goldberg DI, Khoo JC (1990)
Stimulation of a neutral cholesteryl ester hydrolase by cAMP system in P388D1 macrophages
Biochimica & Biophysica Acta
1042 :132
Goldberg DI, Khoo JC (1990)
Biochimica & Biophysica Acta
1042 :132