Title : Biosensor based on acetylcholinesterase immobilized onto layered double hydroxides for flow injection\/amperometric detection of organophosphate pesticides - Gong_2013_Biosens.Bioelectron_39_320 |
Author(s) : Gong J , Guan Z , Song D |
Ref : Biosensors & Bioelectronics , 39 :320 , 2013 |
Abstract :
We developed a highly sensitive flow injection/amperometric biosensor for the detection of organophosphate pesticides (OPs) using layered double hydroxides (LDHs) as the immobilization matrix of acetylcholinesterase (AChE). LDHs provided a biocompatible microenvironment to keep the bioactivity of AChE, due to the intrinsic properties of LDHs (such as a regular structure, good mechanical, chemical and thermal stabilities, and swelling properties). By integrating the flow injection analysis (FIA) with amperometric detection, the resulting AChE-LDHs modified electrode greatly catalyzed the oxidation of the enzymatically generated thiocholine product, and facilitated the detection automation, thus increasing the detection sensitivity. The analytical conditions for the FIA/amperometric detection of OPs were optimized by using methyl parathion (MP) as a model. The inhibition of MP was proportional to its concentration ranging from 0.005 to 0.3ug mL(-1) and 0.3 to 4.0ug mL(-1) with a detection limit 0.6ng mL(-1) (S/N=3). The developed biosensor exhibited good reproducibility and acceptable stability. |
PubMedSearch : Gong_2013_Biosens.Bioelectron_39_320 |
PubMedID: 22868055 |
Gong J, Guan Z, Song D (2013)
Biosensor based on acetylcholinesterase immobilized onto layered double hydroxides for flow injection\/amperometric detection of organophosphate pesticides
Biosensors & Bioelectronics
39 :320
Gong J, Guan Z, Song D (2013)
Biosensors & Bioelectronics
39 :320