Gonzalez_2015_Comp.Biochem.Physiol.A.Mol.Integr.Physiol_182_1

Reference

Title : Effects of 5alpha-dihydrotestosterone on expression of genes related to steroidogenesis and spermatogenesis during the sex determination and differentiation periods of the pejerrey, Odontesthes bonariensis - Gonzalez_2015_Comp.Biochem.Physiol.A.Mol.Integr.Physiol_182_1
Author(s) : Gonzalez A , Fernandino JI , Somoza GM
Ref : Comparative Biochemistry & Physiology A Mol Integr Physiol , 182 :1 , 2015
Abstract :

Sex steroid hormones are important players in the control of sex differentiation by regulating gonadal development in teleosts. Although estrogens are clearly associated with the ovarian differentiation in teleosts, the effects of androgens on early gonadal development are still a matter of debate. Traditionally, 11-ketotestosterone (11-KT) is considered the major androgen in fish; however, 5alpha-dihydrotestosterone (5alpha-DHT), the most potent androgen in tetrapods, was recently found in fish testis and plasma, but its physiological role is still unknown. In this context, the expression of genes associated with steroidogenesis and spermatogenesis, body growth and sex differentiation were assessed in Odontesthes bonariensis larvae fed with food supplemented with two doses of 5alpha-DHT (0.1 and 10mug/g of food) from hatching to 6weeks of age. At the lowest dose, 5alpha-DHT treated larvae showed an estrogenic gene expression pattern, with low hsd11b2 and high cyp19a1a and er2 expression levels with no differences in sex ratio. At the highest dose, 5alpha-DHT produced a male-shifted sex ratio and the larvae exhibited a gene expression profile characteristic of an advancement of spermatogenesis, with inhibition of amh and stimulation of ndrg3. No differences were observed in somatic growth. These results suggest that in this species, 5alpha-DHT could have a role on sex differentiation and its effects can differ according to the dose.

PubMedSearch : Gonzalez_2015_Comp.Biochem.Physiol.A.Mol.Integr.Physiol_182_1
PubMedID: 25499236

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Citations formats

Gonzalez A, Fernandino JI, Somoza GM (2015)
Effects of 5alpha-dihydrotestosterone on expression of genes related to steroidogenesis and spermatogenesis during the sex determination and differentiation periods of the pejerrey, Odontesthes bonariensis
Comparative Biochemistry & Physiology A Mol Integr Physiol 182 :1

Gonzalez A, Fernandino JI, Somoza GM (2015)
Comparative Biochemistry & Physiology A Mol Integr Physiol 182 :1