| Title : Nicotinic acetylcholine receptor: a structural model for alpha-subunit peptide 188-201, the putative binding site for cholinergic agents - Gotti_1988_FEBS.Lett_228_118 |
| Author(s) : Gotti C , Frigerio F , Bolognesi M , Longhi R , Racchetti G , Clementi F |
| Ref : FEBS Letters , 228 :118 , 1988 |
|
Abstract :
A peptide corresponding to amino acid sequence 188-201 of the alpha-subunit of Torpedo AChR binds alpha-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds alpha-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated. |
| PubMedSearch : Gotti_1988_FEBS.Lett_228_118 |
| PubMedID: 3342868 |
Gotti C, Frigerio F, Bolognesi M, Longhi R, Racchetti G, Clementi F (1988)
Nicotinic acetylcholine receptor: a structural model for alpha-subunit peptide 188-201, the putative binding site for cholinergic agents
FEBS Letters
228 :118
Gotti C, Frigerio F, Bolognesi M, Longhi R, Racchetti G, Clementi F (1988)
FEBS Letters
228 :118