Gou_2012_Plant.Cell_24_50

Reference

Title : Acetylesterase-mediated deacetylation of pectin impairs cell elongation, pollen germination, and plant reproduction - Gou_2012_Plant.Cell_24_50
Author(s) : Gou JY , Miller LM , Hou G , Yu XH , Chen XY , Liu CJ
Ref : Plant Cell , 24 :50 , 2012
Abstract :

Pectin is a major component of the primary cell wall of higher plants. Some galacturonyl residues in the backbone of pectinaceous polysaccharides are often O-acetylated at the C-2 or C-3 position, and the resulting acetylesters change dynamically during the growth and development of plants. The processes involve both enzymatic acetylation and deacetylation. Through genomic sequence analysis, we identified a pectin acetylesterase (PAE1) from black cottonwood (Populus trichocarpa). Recombinant Pt PAE1 exhibited preferential activity in releasing the acetate moiety from sugar beet (Beta vulgaris) and potato (Solanum tuberosum) pectin in vitro. Overexpressing Pt PAE1 in tobacco (Nicotiana tabacum) decreased the level of acetyl esters of pectin but not of xylan. Deacetylation engendered differential changes in the composition and/or structure of cell wall polysaccharides that subsequently impaired the cellular elongation of floral styles and filaments, the germination of pollen grains, and the growth of pollen tubes. Consequently, plants overexpressing PAE1 exhibited severe male sterility. Furthermore, in contrast to the conventional view, PAE1-mediated deacetylation substantially lowered the digestibility of pectin. Our data suggest that pectin acetylesterase functions as an important structural regulator in planta by modulating the precise status of pectin acetylation to affect the remodeling and physiochemical properties of the cell wall's polysaccharides, thereby affecting cell extensibility.

PubMedSearch : Gou_2012_Plant.Cell_24_50
PubMedID: 22247250
Gene_locus related to this paper: poptr-b9n0n1

Related information

Gene_locus poptr-b9n0n1

Citations formats

Gou JY, Miller LM, Hou G, Yu XH, Chen XY, Liu CJ (2012)
Acetylesterase-mediated deacetylation of pectin impairs cell elongation, pollen germination, and plant reproduction
Plant Cell 24 :50

Gou JY, Miller LM, Hou G, Yu XH, Chen XY, Liu CJ (2012)
Plant Cell 24 :50