Title : Molecular cloning and characterization of the met2 gene from Ascobolus immersus - Goyon_1988_Gene_63_297 |
Author(s) : Goyon C , Faugeron G , Rossignol JL |
Ref : Gene , 63 :297 , 1988 |
Abstract :
We have cloned the met2 gene from Ascobolus immersus by heterologous hybridization with the MET2 gene of Saccharomyces cerevisiae. This gene codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The complete nucleotide sequence of a 2910-bp DNA fragment carrying the met2 gene has been determined. The gene contains a 165-bp intron which is similar in structure to other fungal introns. The deduced amino acid (aa) sequence (518 aa residues; Mr of 57726) shows three domains with a significant level of homology with the corresponding yeast protein. Northern-blot analysis reveals at least two transcripts (2.4 and 2.1 kb) probably due to transcription termination heterogeneity, as suggested by S1-mapping experiments. Polymorphism has been observed in the met2 gene flanking regions of Ascobolus strains from two different stocks. |
PubMedSearch : Goyon_1988_Gene_63_297 |
PubMedID: 2838393 |
Gene_locus related to this paper: ascim-met2 |
Gene_locus | ascim-met2 |
Goyon C, Faugeron G, Rossignol JL (1988)
Molecular cloning and characterization of the met2 gene from Ascobolus immersus
Gene
63 :297
Goyon C, Faugeron G, Rossignol JL (1988)
Gene
63 :297