| Title : Cortical ChAT(+) neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific manner - Granger_2020_Elife_9_ |
| Author(s) : Granger AJ , Wang W , Robertson K , El-Rifai M , Zanello AF , Bistrong K , Saunders A , Chow BW , Nunez V , Turrero Garcia M , Harwell CC , Gu C , Sabatini BL |
| Ref : Elife , 9 : , 2020 |
| Abstract : The mouse cerebral cortex contains neurons that express choline acetyltransferase (ChAT) and are a potential local source of acetylcholine. However, the neurotransmitters released by cortical ChAT(+) neurons and their synaptic connectivity are unknown. We show that the nearly all cortical ChAT(+) neurons in mice are specialized VIP(+) interneurons that release GABA strongly onto other inhibitory interneurons and acetylcholine sparsely onto layer 1 interneurons and other VIP(+)/ChAT(+) interneurons. This differential transmission of ACh and GABA based on the postsynaptic target neuron is reflected in VIP(+)/ChAT(+) interneuron pre-synaptic terminals, as quantitative molecular analysis shows that only a subset of these are specialized to release acetylcholine. In addition, we identify a separate, sparse population of non-VIP ChAT(+) neurons in the medial prefrontal cortex with a distinct developmental origin that robustly release acetylcholine in layer 1. These results demonstrate both cortex-region heterogeneity in cortical ChAT(+) interneurons and target-specific co-release of acetylcholine and GABA. |
| ESTHER : Granger_2020_Elife_9_ |
| PubMedSearch : Granger_2020_Elife_9_ |
| PubMedID: 32613945 |
Granger AJ, Wang W, Robertson K, El-Rifai M, Zanello AF, Bistrong K, Saunders A, Chow BW, Nunez V, Turrero Garcia M, Harwell CC, Gu C, Sabatini BL (2020)
Cortical ChAT(+) neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific manner
Elife
9 :
Granger AJ, Wang W, Robertson K, El-Rifai M, Zanello AF, Bistrong K, Saunders A, Chow BW, Nunez V, Turrero Garcia M, Harwell CC, Gu C, Sabatini BL (2020)
Elife
9 :