Gray_1999_J.Neurochem_72_1981

Reference

Title : A nitric oxide\/cyclic GMP-dependent protein kinase pathway alters transmitter release and inhibition by somatostatin at a site downstream of calcium entry - Gray_1999_J.Neurochem_72_1981
Author(s) : Gray DB , Polo-Parada L , Pilar GR , Eang P , Metzger RR , Klann E , Meriney SD
Ref : Journal of Neurochemistry , 72 :1981 , 1999
Abstract :

We have examined the somatostatin-mediated modulation of acetylcholine release from intact chick embryo choroid tissue and compared these data with those obtained using acutely dissociated neuronal cell bodies from the chick ciliary ganglion. Acetylcholine release, evoked in a calcium-dependent manner by a high potassium (55 mM KCI) stimulation in both preparations, was inhibited almost completely by 100 nM somatostatin. Measurement of intracellular calcium in these neurons revealed that somatostatin blocked the large calcium transient that was observed in control neurons following KCI exposure. The modulatory effect of somatostatin on transmitter release was significantly attenuated by pre-treatment with pharmacologic agents that selectively block cyclic GMP (cGMP)-dependent protein kinase (PKG) or nitric oxide (NO) synthase. It is interesting that this prevention of somatostatin-mediated acetylcholine release inhibition occurred without reversal of the somatostatin-mediated block of the KCl-evoked calcium transient. Furthermore, a NO donor or cGMP analogue could block KCI-evoked acetylcholine release, but only cGMP could reduce the KCI-evoked calcium transient. Although cGMP could reduce the KCI-evoked calcium transient, a cGMP analogue was shown to reduce calcium ionophore-evoked transmitter release. Thus, somatostatin reduces acetylcholine release by modulating calcium influx, but the NO-PKG pathway can inhibit acetylcholine release, and alter somatostatin-mediated inhibition, by affecting transmitter release at some point after calcium entry.

PubMedSearch : Gray_1999_J.Neurochem_72_1981
PubMedID: 10217275

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Citations formats

Gray DB, Polo-Parada L, Pilar GR, Eang P, Metzger RR, Klann E, Meriney SD (1999)
A nitric oxide\/cyclic GMP-dependent protein kinase pathway alters transmitter release and inhibition by somatostatin at a site downstream of calcium entry
Journal of Neurochemistry 72 :1981

Gray DB, Polo-Parada L, Pilar GR, Eang P, Metzger RR, Klann E, Meriney SD (1999)
Journal of Neurochemistry 72 :1981