| Title : Stereoselectivity of acetylcholinesterase, arylester hydrolase, and chymotrypsin toward 4-nitrophenyl alkyl(phenyl)phosphinates - Grothusen_1986_Pestic.Biochem.Physiol_26_100 |
| Author(s) : Grothusen JR , Brown TM |
| Ref : Pesticide Biochemistry and Physiology , 26 :100 , 1986 |
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Abstract :
Acetylcholinesterases from electric eel and from bovine erythrocytes were inhibited stereoselectively by P(+) 4-nitrophenyl ethyl(phenyl)phosphinate (EPP) and P(+) 4-nitrophenyl isopropyl(phenyl)phosphinate (IPP). Bovine pancreatic alpha-chymotrypsin was inhibited stereoselectively also, but the P(-) enantiomers of both EPP and IPP were more active than P(+). Arylester hydrolase was nine times more active in the hydrolysis of P(-) EPP than P(+). Chiral-phase HPLC was employed to isolate the enantiomers from racemic mixture for experiments and to analyze reaction mixtures initiated with racemic EPP or IPP. |
| PubMedSearch : Grothusen_1986_Pestic.Biochem.Physiol_26_100 |
| PubMedID: |
Grothusen JR, Brown TM (1986)
Stereoselectivity of acetylcholinesterase, arylester hydrolase, and chymotrypsin toward 4-nitrophenyl alkyl(phenyl)phosphinates
Pesticide Biochemistry and Physiology
26 :100
Grothusen JR, Brown TM (1986)
Pesticide Biochemistry and Physiology
26 :100