Title : Characteristics of a recombinant Fusarium verticillioides cutinase and its effects on enzymatic hydrolysis of rice straw - Gu_2021_Int.J.Biol.Macromol_171_382 |
Author(s) : Gu S , Liu C , Zhang W , Qu M , Li Y , Zang Y , Xiong X , Pan K , Zhao X |
Ref : Int J Biol Macromol , 171 :382 , 2021 |
Abstract :
The current study heterologously expressed a cutinase from Fusarium verticillioides by Pichia pastoris and investigated its properties and effects on the hydrolysis of rice straw. The optimal pH and temperature for F. verticillioides cutinase were 8.0 and 50 degreesC, respectively. F. verticillioides cutinase had poor thermal stability and could be inhibited by some metal ions, inhibitors, and detergents (5 mM), including Ni(2+), Zn(2+), Cu(2+), Ca(2+), Mn(2+), sodium dodecyl sulfate, EDTA, and Tween-20. F. verticillioides cutinase could tolerate 15% methanol and dimethyl sulfoxide but was significantly repressed by 15% ethanol and acetone with 48% and 63% residual activity, respectively. F. verticillioides cutinase could degrade the cuticle of rice straw with palmitic acid and stearic acid as the main products. However, the dissolving sugars released from the rice straw treated with F. verticillioides cutinase were significantly reduced by 29.2 microg/mL compared with the control (107.9 microg/mL). Similarly, the reducing sugars produced from the cellulase hydrolysis of rice straw pretreated with F. verticillioides cutinase were reduced by 63.5 microg/mL relative to the control (253.6 microg/mL). Scanning electron microscopy results showed that numerous tuberculate or warty protrusions were present nearly everywhere on the surface of rice straw treated with F. verticillioides cutinase, and some protrusions even covered and blocked the stomata of the rice straw surface. Current limited data indicate that F. verticillioides cutinase might not be an appropriate choice for improving the utilization of agricultural straws. |
PubMedSearch : Gu_2021_Int.J.Biol.Macromol_171_382 |
PubMedID: 33434547 |
Gene_locus related to this paper: gibm7-w7lbp5 |
Gene_locus | gibm7-w7lbp5 |
Gu S, Liu C, Zhang W, Qu M, Li Y, Zang Y, Xiong X, Pan K, Zhao X (2021)
Characteristics of a recombinant Fusarium verticillioides cutinase and its effects on enzymatic hydrolysis of rice straw
Int J Biol Macromol
171 :382
Gu S, Liu C, Zhang W, Qu M, Li Y, Zang Y, Xiong X, Pan K, Zhao X (2021)
Int J Biol Macromol
171 :382