Guercio_2022_Commun.Biol_5_126

Reference

Title : Structural and functional analyses explain Pea KAI2 receptor diversity and reveal stereoselective catalysis during signal perception - Guercio_2022_Commun.Biol_5_126
Author(s) : Guercio AM , Torabi S , Cornu D , Dalmais M , Bendahmane A , Le Signor C , Pillot JP , Le Bris P , Boyer FD , Rameau C , Gutjahr C , de Saint Germain A , Shabek N
Ref : Commun Biol , 5 :126 , 2022
Abstract :

KAI2 proteins are plant alpha/beta hydrolase receptors which perceive smoke-derived butenolide signals and endogenous, yet unidentified KAI2-ligands (KLs). The number of functional KAI2 receptors varies among species and KAI2 gene duplication and sub-functionalization likely plays an adaptative role by altering specificity towards different KLs. Legumes represent one of the largest families of flowering plants and contain many agronomic crops. Prior to their diversification, KAI2 underwent duplication resulting in KAI2A and KAI2B. Here we demonstrate that Pisum sativum KAI2A and KAI2B are active receptors and enzymes with divergent ligand stereoselectivity. KAI2B has a higher affinity for and hydrolyses a broader range of substrates including strigolactone-like stereoisomers. We determine the crystal structures of PsKAI2B in apo and butenolide-bound states. The biochemical, structural, and mass spectra analyses of KAI2s reveal a transient intermediate on the catalytic serine and a stable adduct on the catalytic histidine, confirming its role as a bona fide enzyme. Our work uncovers the stereoselectivity of ligand perception and catalysis by diverged KAI2 receptors and proposes adaptive sensitivity to KAR/KL and strigolactones by KAI2B.

PubMedSearch : Guercio_2022_Commun.Biol_5_126
PubMedID: 35149763
Gene_locus related to this paper: pea-KAI2A , pea-KAI2B

Related information

Inhibitor Hydroxy-D-ring
Substrate Hydroxy-D-ring    GR24
Gene_locus Hydroxy-D-ring    GR24    pea-KAI2A    pea-KAI2B
Gene_locus_frgt Hydroxy-D-ring    GR24    pea-KAI2A    pea-KAI2B    pea-KAI2C
Structure Hydroxy-D-ring    GR24    pea-KAI2A    pea-KAI2B    pea-KAI2C    7K2Z    7K38

Citations formats

Guercio AM, Torabi S, Cornu D, Dalmais M, Bendahmane A, Le Signor C, Pillot JP, Le Bris P, Boyer FD, Rameau C, Gutjahr C, de Saint Germain A, Shabek N (2022)
Structural and functional analyses explain Pea KAI2 receptor diversity and reveal stereoselective catalysis during signal perception
Commun Biol 5 :126

Guercio AM, Torabi S, Cornu D, Dalmais M, Bendahmane A, Le Signor C, Pillot JP, Le Bris P, Boyer FD, Rameau C, Gutjahr C, de Saint Germain A, Shabek N (2022)
Commun Biol 5 :126