| Title : A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode (Bursaphelenchus xylophilus) - Guo_2017_Pestic.Biochem.Physiol_135_64 |
| Author(s) : Guo Q , Du G , Qi H , Zhang Y , Yue T , Wang J , Li R |
| Ref : Pestic Biochem Physiol , 135 :64 , 2017 |
|
Abstract :
The ethanol extract of Punica granatum L. rind was tested to show significant nematicidal activity against pine wood nematode. Three nematicidal compounds were obtained from the ethanol extract by bioassay-guided fractionation and identified as punicalagin 1, punicalin 2, and corilagin 3 by mass and nuclear magnetic resonance spectral data analysis. Punicalagin 1 was most active against PWN among the purified compounds with the LC50 value of 307.08muM in 72h. According to the enzyme assays in vitro, punicalagin 1 could inhibit the activity of acetylcholinesterase, amylase and cellulase from PWN with IC50 value of 0.60mM, 0.96mM and 1.24mM, respectively. The morphological structures of PWNs treated by punicalagin 1 were greatly changed. These physiological effects of punicalagin 1 on PWN may helpful to elucidate its nematicidal mechanism. |
| PubMedSearch : Guo_2017_Pestic.Biochem.Physiol_135_64 |
| PubMedID: 28043333 |
Guo Q, Du G, Qi H, Zhang Y, Yue T, Wang J, Li R (2017)
A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode (Bursaphelenchus xylophilus)
Pestic Biochem Physiol
135 :64
Guo Q, Du G, Qi H, Zhang Y, Yue T, Wang J, Li R (2017)
Pestic Biochem Physiol
135 :64