| Title : Liquid chromatographic determination of tacrine and its metabolites in rat bile microdialysates - Hadwiger_1994_J.Chromatogr.B.Biomed.Appl_655_235 |
| Author(s) : Hadwiger ME , Telting-Diaz M , Lunte CE |
| Ref : Journal of Chromatography B Biomed Appl , 655 :235 , 1994 |
| Abstract : A microbore liquid chromatographic method using a phenyl stationary phase was developed for the determination of 9-amino-1,2,3,4-tetrahydroacridine (tacrine, THA) and its metabolites in microdialysis samples of bile. Analysis of microdialysis samples requires analytical methods with low detection limits and small sample volume requirements. The method uses a 1-mm I.D. phenyl column and fluorescence detection. A detection limit of 0.25 ng/ml in a 5-microliters sample was achieved for THA. This method was then used to determine THA and THA-1-ol in the bile dialysate of a rat. Because of the small sample volume requirements, a 10-min temporal resolution was achieved for the microdialysis experiment. The low detection limit allowed the THA concentration in the bile to be monitored for more than 4 h following a 1.0 mg/kg i.v. dose of THA. |
| ESTHER : Hadwiger_1994_J.Chromatogr.B.Biomed.Appl_655_235 |
| PubMedSearch : Hadwiger_1994_J.Chromatogr.B.Biomed.Appl_655_235 |
| PubMedID: 8081469 |
Hadwiger ME, Telting-Diaz M, Lunte CE (1994)
Liquid chromatographic determination of tacrine and its metabolites in rat bile microdialysates
Journal of Chromatography B Biomed Appl
655 :235
Hadwiger ME, Telting-Diaz M, Lunte CE (1994)
Journal of Chromatography B Biomed Appl
655 :235