| Title : Design and synthesis of an ER-specific fluorescent probe based on carboxylesterase activity with quinone methide cleavage process - Hakamata_2011_Bioorg.Med.Chem.Lett_21_3206 |
| Author(s) : Hakamata W , Machida A , Oku T , Nishio T |
| Ref : Bioorganic & Medicinal Chemistry Lett , 21 :3206 , 2011 |
|
Abstract :
CEs are important enzymes that catalyze the hydrolysis of prodrugs. In this Letter, we present a new mechanistic ER-specific fluorescent probe 1 based on CE activity. Permeation of 1 into cells and subsequent hydrolytic activation by CEs causes spontaneously quinone methide cleavage, resulting in bright red fluorescence in ER with high specificity. Probe 1 was developed for CE activity imaging and inhibitor screening at the cellular level. |
| PubMedSearch : Hakamata_2011_Bioorg.Med.Chem.Lett_21_3206 |
| PubMedID: 21549595 |
Hakamata W, Machida A, Oku T, Nishio T (2011)
Design and synthesis of an ER-specific fluorescent probe based on carboxylesterase activity with quinone methide cleavage process
Bioorganic & Medicinal Chemistry Lett
21 :3206
Hakamata W, Machida A, Oku T, Nishio T (2011)
Bioorganic & Medicinal Chemistry Lett
21 :3206