Title : Genomic DNA analysis of genes encoding (hemi-)cellulolytic enzymes of the anaerobic fungus Piromyces sp. E2 - Harhangi_2003_Gene_314_73 |
Author(s) : Harhangi HR , Akhmanova A , Steenbakkers PJ , Jetten MS , van der Drift C , Op den Camp HJ |
Ref : Gene , 314 :73 , 2003 |
Abstract :
Anaerobic fungi contain more than one copy of genes encoding (hemi-)cellulases in their genome. The arrangement of these genes on the chromosomes was not known. A genomic DNA (gDNA) library of Piromyces sp. E2 was screened with different probes specific for (hemi-)cellulolytic enzymes. This screening resulted in three gDNA clones with genes encoding glycoside hydrolase enzymes of families 1 (beta-glucosidase), 6 (exoglucanase) and 26 (mannanase). Each clone contained two or more genes of the same family. Comparison of the gene copies on a clone revealed that they were highly homologous, and in addition, 54-75% of the substitutions was synonymous. One of the mannanase genes contained an intron. PCR with selected primers resulted in a gDNA clone with a new representative (cel9B) of glycoside hydrolase family 9 (endoglucanase). Comparison with cel9A revealed that cel9B had 67% homology on the nucleotide level. Furthermore, three introns were present. All results of this paper taken together provided evidence for duplications of (hemi-)cellulolytic genes, which resulted in clusters of almost identical genes arranged head-to-tail on the genome. In contrast to other eukaryotes, this phenomenon appears frequently in anaerobic fungi. |
PubMedSearch : Harhangi_2003_Gene_314_73 |
PubMedID: 14527719 |
Gene_locus_frgt | pirsp-Q870B0 |
Harhangi HR, Akhmanova A, Steenbakkers PJ, Jetten MS, van der Drift C, Op den Camp HJ (2003)
Genomic DNA analysis of genes encoding (hemi-)cellulolytic enzymes of the anaerobic fungus Piromyces sp. E2
Gene
314 :73
Harhangi HR, Akhmanova A, Steenbakkers PJ, Jetten MS, van der Drift C, Op den Camp HJ (2003)
Gene
314 :73