| Title : Muscarinic signalling affects intracellular calcium concentration during the first cell cycle of sea urchin embryos - Harrison_2002_Cell.Calcium_31_289 |
| Author(s) : Harrison PK , Falugi C , Angelini C , Whitaker MJ |
| Ref : Cell Calcium , 31 :289 , 2002 |
| Abstract : The existence of a response to acetylcholine (ACh) and cholinomimetic drugs in sea urchin eggs and zygotes was investigated in two sea urchin species: Paracentrotus lividus and Lytechinus pictus. The calcium sensitive fluorescent probe, Fura-2 dextran, was employed to investigate the regulation of cytosolic free calcium concentration ([Ca(2+)](i)) by cholinomimetic drugs in unfertilised and fertilised eggs of both the sea urchin species. Exposure to cholinomimetic agonists/antagonists, either extracellularly or intracellularly, had no effect either on resting [Ca(2+)](i) levels in the unfertilised sea urchin egg, or on the transient [Ca(2+)](i) increase at fertilisation. However, following fertilisation, extracellular application of ACh receptors agonists, such as ACh and carbachol, predominantly muscarinic agonist, but not nicotine, induced a significant increase in [Ca(2+)](i), which was partially inhibited by atropine. As a consequence of exposure after fertilisation to the agonists of ACh receptors, chromatin structure was transiently affected. The hypothesis is proposed that muscarinic receptors may be involved in the (presumably Ca(2+)-dependent) modulation of the nuclear status during the first cell cycles. |
| ESTHER : Harrison_2002_Cell.Calcium_31_289 |
| PubMedSearch : Harrison_2002_Cell.Calcium_31_289 |
| PubMedID: 12098218 |
Harrison PK, Falugi C, Angelini C, Whitaker MJ (2002)
Muscarinic signalling affects intracellular calcium concentration during the first cell cycle of sea urchin embryos
Cell Calcium
31 :289
Harrison PK, Falugi C, Angelini C, Whitaker MJ (2002)
Cell Calcium
31 :289