| Title : Extracellular carbohydrate esterase from the basidiomycete Coprinopsis cinerea released ferulic and acetic acids from xylan - Hashimoto_2010_Biosci.Biotechnol.Biochem_74_1722 |
| Author(s) : Hashimoto K , Kaneko S , Yoshida M |
| Ref : Biosci Biotechnol Biochem , 74 :1722 , 2010 |
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Abstract :
cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, alpha-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-beta-1,4-xylanase. |
| PubMedSearch : Hashimoto_2010_Biosci.Biotechnol.Biochem_74_1722 |
| PubMedID: 20699557 |
Hashimoto K, Kaneko S, Yoshida M (2010)
Extracellular carbohydrate esterase from the basidiomycete Coprinopsis cinerea released ferulic and acetic acids from xylan
Biosci Biotechnol Biochem
74 :1722
Hashimoto K, Kaneko S, Yoshida M (2010)
Biosci Biotechnol Biochem
74 :1722