He_2012_Bioresour.Technol_114_1

Reference

Title : Lipase-mediated synthesis of water-soluble plant stanol derivatives in tert-butanol - He_2012_Bioresour.Technol_114_1
Author(s) : He WS , Li JJ , Pan XX , Zhou Y , Jia CS , Zhang XM , Feng B
Ref : Bioresour Technol , 114 :1 , 2012
Abstract :

The effects of solvents with different log P values, and of lipases on the synthesis of water-soluble plant stanol derivatives were investigated. Results showed that conversion in solvents with log P<0.37 was mainly controlled by the hydrophobicity of the solvent and subsequent complete or partial deactivation of the enzyme. The solubility of substrate was the leading factor for the conversion in solvents with log P>0.37. Lipozyme RM IM and tert-butanol was the most suitable biocatalyst and solvent, respectively. The highest yield (>51%) of plant stanyl sorbitol succinate was obtained under the selected conditions: 50 mol/mL plant stanyl hemisuccinate, 1:3 molar ratio of plant stanyl hemisuccinate to d-sorbitol, 80 mg/mL 3 molecular sieves and 100mg/mL Lipozyme RM IM in tert-butanol, 150 r/min and 55 degreeC. Fourier transform infrared spectroscopy, mass spectroscopy and nuclear magnetic resonance spectroscopy were adopted to determine the structure of product, suggesting that water-soluble plant stanol derivatives were successfully synthesized.

PubMedSearch : He_2012_Bioresour.Technol_114_1
PubMedID: 22464062

Related information

Substrate Sorbitol

Citations formats

He WS, Li JJ, Pan XX, Zhou Y, Jia CS, Zhang XM, Feng B (2012)
Lipase-mediated synthesis of water-soluble plant stanol derivatives in tert-butanol
Bioresour Technol 114 :1

He WS, Li JJ, Pan XX, Zhou Y, Jia CS, Zhang XM, Feng B (2012)
Bioresour Technol 114 :1