| Title : Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli - Henne_2000_Appl.Environ.Microbiol_66_3113 |
| Author(s) : Henne A , Schmitz RA , Bomeke M , Gottschalk G , Daniel R |
| Ref : Applied Environmental Microbiology , 66 :3113 , 2000 |
|
Abstract :
Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized. |
| PubMedSearch : Henne_2000_Appl.Environ.Microbiol_66_3113 |
| PubMedID: 10877816 |
| Gene_locus related to this paper: bacte-ester , gamho-LIPA , uncba-Q9KIU0 , uncba-Q9KIU2 |
| Gene_locus | bacte-ester gamho-LIPA uncba-Q9KIU0 uncba-Q9KIU2 |
Henne A, Schmitz RA, Bomeke M, Gottschalk G, Daniel R (2000)
Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli
Applied Environmental Microbiology
66 :3113
Henne A, Schmitz RA, Bomeke M, Gottschalk G, Daniel R (2000)
Applied Environmental Microbiology
66 :3113