Henriksen_1997_Toxicon_35_901

Reference

Title : Detection of an anatoxin-a(s)-like anticholinesterase in natural blooms and cultures of cyanobacteria\/blue-green algae from Danish lakes and in the stomach contents of poisoned birds - Henriksen_1997_Toxicon_35_901
Author(s) : Henriksen P , Carmichael WW , An J , Moestrup O
Ref : Toxicon , 35 :901 , 1997
Abstract :

Ten natural bloom samples of cyanobacteria from the Danish lakes Knud so (5), Ravn so (4), and Salten Langso (1) collected during 1993-1995 were assayed for toxicity by mouse bioassay, for acetylcholinesterase inhibiting activity by a colorimetric method, and for microcystins by enzyme-linked immunosorbent assay. In the mouse bioassay, seven samples were neurotoxic, two were non-toxic and one gave a protracted toxic response. One of the non-toxic and the single protracted toxic sample both contained anticholinesterase activity equivalent to 4 micrograms anatoxin-a(s) g-1. The neurotoxic samples contained equivalents to 20-3300 micrograms anatoxin-a(s) g-1. The highest anticholinesterase activities (equivalent to 2300 and 3300 micrograms anatoxin-a(s) g-1, respectively) were found in samples collected from Lake Knud so in connection with bird-kills in 1993 and 1994. Small amounts of microcystins (0.1-0.9 microgram g-1) were detected in all samples but one. All Lake Knud so and Lake Ravn so samples were dominated by Anabaena lemmermannii, and the Lake Salten Langso sample by several species of Anabaena. Gel filtration profiles indicated similarity between the toxic component from the Lake Knud so 1994 bloom with registered bird-kills and anatoxin-a(s) isolated from Anabaena flos-aquae NRC-525-17. Anticholinesterase-producing cultures of A. lemmermannii were isolated from the Lake Knud so 1993 bloom. These laboratory cultures produced anatoxin-a(s) equivalents of 29-743 micrograms g-1. Other cultures of A. lemmermannii isolated from Lake Knud so and Lake Ravn so were hepatotoxic or non-toxic. Dead birds collected from Lake Knud so during the neurotoxic 1993 Anabaena bloom possibly died from cyanobacterial toxicosis. The stomach contents contained colonies and single trichomes of Anabaena, and anticholinesterase activities equivalent to 2.1-89.7 micrograms anatoxin-a(s) kg-1 body weight and microcystins (53-95 ng kg-1) were also detected.

PubMedSearch : Henriksen_1997_Toxicon_35_901
PubMedID: 9241784

Related information

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Citations formats

Henriksen P, Carmichael WW, An J, Moestrup O (1997)
Detection of an anatoxin-a(s)-like anticholinesterase in natural blooms and cultures of cyanobacteria\/blue-green algae from Danish lakes and in the stomach contents of poisoned birds
Toxicon 35 :901

Henriksen P, Carmichael WW, An J, Moestrup O (1997)
Toxicon 35 :901