Hernandez_2002_Insect.Biochem.Mol.Biol_32_1009

Reference

Title : Allele frequency and gene expression of a putative carboxylesterase-encoding gene in a pyrethroid resistant strain of the tick Boophilus microplus - Hernandez_2002_Insect.Biochem.Mol.Biol_32_1009
Author(s) : Hernandez R , Guerrero FD , George JE , Wagner GG
Ref : Insect Biochemistry & Molecular Biology , 32 :1009 , 2002
Abstract :

We utilized RNA Northern blot analysis and ribonuclease protection assays (RPA) to study the mRNA expression level of a putative carboxylesterase-encoding gene from several strains of Boophilus microplus (Canestrini). Both the Northern analysis and RPAs indicated that an esterase transcript was more abundant in the pyrethroid resistant strain, Coatzacoalcos (Cz), compared to a susceptible control strain and a resistant strain whose pyrethroid resistance is mediated through a target site insensitivity mechanism. A PCR-based assay was designed to identify the presence of a previously reported point mutation in this B. microplus esterase gene. The reported G-->A substitution at nucleotide 1120 creates an EcoR I site in the mutant allele which can be detected by EcoR I digestion of the amplification products. The PCR assays showed that the frequency of the mutant allele was highest in the Cz-resistant strain, which has been shown to have an esterase-mediated resistance mechanism. The PCR assay can be performed either on individual tick larvae or hemolymph from adults.

PubMedSearch : Hernandez_2002_Insect.Biochem.Mol.Biol_32_1009
PubMedID: 12213237

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Citations formats

Hernandez R, Guerrero FD, George JE, Wagner GG (2002)
Allele frequency and gene expression of a putative carboxylesterase-encoding gene in a pyrethroid resistant strain of the tick Boophilus microplus
Insect Biochemistry & Molecular Biology 32 :1009

Hernandez R, Guerrero FD, George JE, Wagner GG (2002)
Insect Biochemistry & Molecular Biology 32 :1009