Title : Automated dibucaine number measurement with DuPont Dimension ES and AR analyzers - Holownia_1995_Clin.Chem_41_664 |
Author(s) : Holownia P , Newman DJ , Bruno C , LaGamba P , Gerrits M , Salemink G , Ossani M , Price CP |
Ref : Clinical Chemistry , 41 :664 , 1995 |
Abstract :
We report a fully automated method for determining dibucaine number (DN) in a single-run procedure involving Dimension cholinesterase (CHE) Flex pseudo-(P)CHE reagents. The method was developed and optimized with the "open channels" and "kinetic" software facilities of the Dimension-ES instrument, where the DN is calculated automatically by an algorithm from the ratio of the uninhibited and inhibited rates, measured bichromatically, from a single analysis. The protocol was satisfactorily assessed for substrate depletion, linearity, reagent stability. and the effects of different dibucaine concentrations. Validation was performed across a range of CHE activities (1.5-22 kU/L) representing the three main genotypes, UU, UA, and AA. The respective DNs (mean +/- SD), determined on the Dimension-ES, were 82.0 +/- 1.6 (n = 32), 71.0 +/- 3.1 (n = 10), and 23.0 +/- 2.7 (n = 14), with corresponding imprecisions (CV) of 0.3%, 0.6%, and 5.2% (intraassay) and 0.7%, 0.7%, and 8.6% (interassay). Comparisons with reference (x) laboratory values and the DuPont aca (x') procedure (n = 53) gave regression equations of: y = 0.88x + 11.2, r = 0.99, and y = 0.85x' + 11.9, r = 0.99. A separate trial conducted with a Dimension-AR instrument gave similar performances. We conclude that the new DN method is fast, efficient, and appropriate for clinical use. |
PubMedSearch : Holownia_1995_Clin.Chem_41_664 |
PubMedID: 7729043 |
Holownia P, Newman DJ, Bruno C, LaGamba P, Gerrits M, Salemink G, Ossani M, Price CP (1995)
Automated dibucaine number measurement with DuPont Dimension ES and AR analyzers
Clinical Chemistry
41 :664
Holownia P, Newman DJ, Bruno C, LaGamba P, Gerrits M, Salemink G, Ossani M, Price CP (1995)
Clinical Chemistry
41 :664